Chromatographic Separation and Analysis of Naphazoline and Its Hydrolytic Degradation Products**Columbia University College of Pharmacy, New York, N. Y.

✍ Scribed by Schwartz, Michael A. ;Kuramoto, Roy ;Malspeis, Louis

• Publisher: Elsevier
• Year: 1956
• Weight: 273 KB
• Volume: 45
• Category: Article
• ISSN: 0095-9553
• DOI: 10.1002/jps.3030451214

✦ Synopsis

n-heptane; n-butanol, Baker analyzed reagent; ethyl acetate, Baker analyzed reagent; buffer solution, pH 8.5,75 ml. 0.2 M boric acid plus 25 d. 0.2 M sodium borate. Equipment.-Beckman Spectrophotometer, Model DU; chromatograph tubes, 400 mm. long, 20 mm. in diameter, constricted at one end into a short delivery tube 7 mm. in diameter. Glass indentations were made near the lower end of the tube to support the column. Preparation of 1-Naphthylacetylethylenediamine. -This was prepared by a modification of the method used by Miescher, et al. (1). Five grams of naphazoline hydrochloride was rduxed with 100 ml. of Wisconsin, Madison 6.