𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Chromatographic resolution of nucleic acids extracted from Penicillium chrysogenum

✍ Scribed by Saunders, Gunter ;Rogers, Mark E. ;Adlard, Maxwell W. ;Holt, Geoffrey

Publisher
Springer
Year
1984
Tongue
English
Weight
360 KB
Volume
194
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

High molecular weight DNA extracted from Penicillium chrysogenum has been fractionated using RPC-5 Analog, into three distinct types designated 1, 2 and 3. Types I and 2 have the same buoyant density of 1.710 g/cm 3 and together appear to comprise the nuclear DNA. Type 1 is enriched for repeated sequences which are normally observed in restriction digests of P. chrysogenum total DNA. Conversely, type 2 appears to be composed entirely of non-repetitive sequences. Type 3 has been identified as mitochondrial DNA, having a buoyant density of 1.695 g/ cm 3 and an estimated molecular weight of 31.6 x 106 Daltons.

πŸ“œ SIMILAR VOLUMES

High-resolution structure of exo-arabina
High-resolution structure of exo-arabinanase from Penicillium chrysogenum
✍ Sogabe, Yuri ;Kitatani, Tomoya ;Yamaguchi, Asako ;Kinoshita, Takayoshi ;Adachi, πŸ“‚ Article πŸ“… 2011 πŸ› International Union of Crystallography 🌐 English βš– 692 KB

Arabinanase Abnx from Penicillium chrysogenum 31B, which belongs to the GH93 family, releases arabinobiose from the nonreducing terminus of Ξ±-1,5-L-arabinan, which is distributed in the primary cell walls of higher plants. Crystal structures of Abnx and of its complex with arabinobiose were determin

Extraction of Nucleic Acid Fragments fro
Extraction of Nucleic Acid Fragments from Gels
✍ Biji T. Kurien; R.Hal Scofield πŸ“‚ Article πŸ“… 2002 πŸ› Elsevier Science 🌐 English βš– 315 KB

Molecular cloning experiments rely greatly on the isolation and analysis of defined DNA fragments. Agarose gel electrophoresis and polyacrylamide gel electrophoresis (reviewed in 1-11) are simple, yet powerful, high-resolution methods of separating specific DNA fragments on the basis of size and con