Chromatographic properties of reversed phase stationary phases under pressure and electro driven conditions. Effect of buffer composition
✍ Scribed by Jan Jiskra; Tao Jiang; Henk A. Claessens; Carel A. Cramers
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 346 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1040-7685
No coin nor oath required. For personal study only.
✦ Synopsis
Four different reversed-phase RP stationary phases CEC Hypersil . C18, Zorbax Rx SIL C18, Zorbax 300 Rx SIL C18 and Zorbax PSM1000rC18 Ž were examined under high-performance liquid chromatographic pressure-driven, . Ž . HPLC , and capillary electrochromatographic electro-driven, CEC conditions using an acetonitrile mobile phase combined with twenty different buffer systems Ž . different cations, anions, pH andror ionic strengths . Chromatographic performance tests under HPLC and CEC conditions were carried out using acidic, basic and neutral polarrnon-polar compounds. Parameters such as plate number, retention factor and asymmetry were used to describe the behavior of the RP-columns under both HPLC and CEC conditions. The buffer systems differently influence chromatographic characteristics of porous RP-phases under CEC and HPLC conditions. Thus the choice of an appropriate buffer can be critical for an application applied on an entire system.