Chromatographic probing of protein-sorbent interactions
✍ Scribed by Viesturs Lesin; Eli Ruckenstein
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 880 KB
- Volume
- 132
- Category
- Article
- ISSN
- 0021-9797
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📜 SIMILAR VOLUMES
A novel mass spectrometry-based methodology using electrospray ionization (ESI) is described for the detection of protein-protein [interferon (IFN)-γ dimer] and protein-ligand [ras-guanosine diphosphate (GDP)] noncovalent interactions. The method utilizes ESI from aqueous solution at appropriate pH.
Considerable effort has been devoted to mapping the complex interactions that make up the molecular circuitry of living cells. For weak or transient interactions that are not easily identified through affinity-based approaches, methods such as yeast two-hybrid screening, protein-fragment compleme