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Chromatographic models to predict the elution of ionizable analytes by organic modifier gradient in reversed phase liquid chromatography

✍ Scribed by Axel Andrés; Adolfo Téllez; Martí Rosés; Elisabeth Bosch

Book ID
116362711
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
729 KB
Volume
1247
Category
Article
ISSN
1873-3778

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## Abstract The use of gradient elution in RP‐HPLC is increasing. Aqueous buffers, such as phosphate at pH 1.7, with acetonitrile as the organic modifier are particularly advantageous as eluents with linear elution strength (LES). In the isocratic mode, under the experimental conditions used, fairl