Chromatographic evaluation and comparison of three β-cyclodextrin-based stationary phases by capillary liquid chromatography and pressure-assisted capillary electrochromatography
✍ Scribed by Bo Lin; Siu-Choon Ng; Yu-Qi Feng
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 206 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0173-0835
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✦ Synopsis
Abstract
Enantiomer separations were performed on three β‐cyclodextrin‐based chiral stationary phases (CSP) containing the pernaphthylcarbamoylated β‐cyclodextrin (CSP 1), peracetylated β‐cyclodextrin (CSP 2) and permethylated β‐cyclodextrin (CSP 3) as chiral selectors by capillary liquid chromatography and pressure‐assisted capillary electrochromatography in this study. Triethylammonium acetate/MeOH or phosphate buffer/MeOH was used as the mobile phase. The experimental factors affecting chiral separations have been examined for each CSP, including pH of the buffers, methanol content and applied voltage. Under optimal separation conditions, a number of racemic compounds were resolved into their enantiomers on three cyclodextrin‐based CSP. A comparative study on the performance of three CSP revealed the presence of carbonyl functional groups as well as aromatic rings in the cyclodextrin derivatives, enhanced the interaction between the analytes and CSP, and thus improved enantioselectivity of the CSP.