Chromatographic behaviour of monoclonal antibodies against wild-type amidase from Pseudomonas aeruginosa on immobilized metal chelates

Screening of suitable immobilized metal

Screening of suitable immobilized metal chelates for adsorption of monoclonal antibodies against mutant amidase from Pseudomonas aeruginosa

✍ Sónia Martins; Jorge Andrade; Amin Karmali; Maria Luísa Serralheiro 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 129 KB

## Abstract The chromatographic behaviour of monoclonal antibodies (MAbs) of IgM class against mutant (T103I) amidase from __Pseudomonas aeruginosa__ was investigated. The effect of ligand concentration, the length of spacer arm and the nature of metal ion were investigated on immobilized metal ion