Chromatographic behavior of underivatized lidocaine and metabolites in CGC

✍ Scribed by Mohamed Abdel-Rehim; Margareta Bielenstein; Torbjörn Arvidsson

• Book ID: 101295489
• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 151 KB
• Volume: 10
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1998)10:7<589::aid-mcs5>3.0.co;2-c

✦ Synopsis

The chromatographic behavior in underivatized form of lidocaine and Ž . six of its metabolites was investigated using capillary gas chromatography CGC . Ten fused silica capillary columns from different manufacturers covering a wide range of polarity were tested. On most of the used columns separation of the analytes was obtained; however, the performance with respect to peak symmetry varied extensively. An improvement in peak symmetry and resolution was observed using HP1 and Rtx-1 columns compared to HT5, despite the fact that all column Ž . types were nonpolar. The highly polar column BPX70 could separate only five of Ž seven compounds. Generally, the intermediate polar columns DB17, HP50, and . BPX35 showed the best performance with respect to peak symmetry, and at optimal conditions it took only 10 min to separate all the metabolites. The effect of Ž . different injector temperatures 200᎐350ЊC on the stability of the compounds studied was also investigated. The minimum detectable quantities for lidocaine and its metabolites were between 30 and 400 fmol using nitrogen phosphorus detection.