Chromatographic and spectroscopic studies on the constituents in male and female flowers of Hagenia abyssinica

✍ Scribed by Tibebe Z. Woldemariam; Anthony F. Fell; Peter A. Linley

• Book ID: 103911400
• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 317 KB
• Volume: 8
• Category: Article
• ISSN: 0731-7085
• DOI: 10.1016/0731-7085(90)80133-a

✦ Synopsis

The phenolic constituents of male and female flowers of Hagenia abyssinica were examined using chromatographic and spectroscopic techniques. Chromatographic analysis of kosins (phloroglucinols) was carried out using TLC, reversed-phase LC and glass-capillary GLC. Purification of the crude kosins was effected by silica gel column chromatography and purification of phenolic acids using ion-exchange resins and preparative TLC on silica gel. The kosins were characterized by TLC, LC, UV, IR, MS and H1 NMR spectroscopy while the phenolic acids were characterized by UV-spectroscopy, TLC and LC. The phenolic acids found were protocatechuic acid, p-hydroxybenzoic acid and vanillic acid. The content of the kosins and phenolic acids was established quantitatively for both male and female flowers.