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Chromatographic analysis of polysaccharides extracted from Chinese indocalamus tesselatus

✍ Scribed by Y. Q. Ding; Y. Li; C. Y. Chen; E. A. Elmahadi; H. B. Xu

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
91 KB
Volume
12
Category
Article
ISSN
0269-3879

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✦ Synopsis

Three fractions of polysaccharides that have high biological activity were extracted from Chinese bamboo leaves (Indocalamus Tesselatus) with 0.9% sodium chloride, 1% ammonium oxalate and 85% ethanol. Their component sugars were investigated by paper chromatography developed with n-butanol-pyridine-water (6:4:3 by vol), and by gas chromatography with crown ester stationary-phase-fused silica capillary column. Their monosaccharide compositions are different from each other with glucuronic acid, galactose, arabinose, mannose, glucose, and xylose (52, 18, 9, 7 and 6%, respectively) in one fraction, only glucuronic acid in another and glucuronic acid, fucose, arabinose and xylose (36, 31, 24 and 9%, respectively) in the third fraction. The protection effect of two polysaccharides against experimental hepatitis was studied in mice.

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