Chromatin structure changes suggest a compensatory response to c-myc gene amplification in malignant fibrous histiocytoma
✍ Scribed by Jane S. Gibson; Byron P. Croker
- Book ID
- 102879797
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 858 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
Changes in chromatin structure as determined from DNAse I hypersensitive site analysis are associated with c-myc amplification and increased transcript/protein levels in malignant fibrous histiocytoma (MFH) cell lines. A DNAse I hypersensitive site near the PO promoter region was observed in one MFH cell line (UR HCL 1 ), and in normal fibroblasts (HFF), but not in an MFH cell line with an amplified c-myc gene (P3C). A DNAse I hypersensitive site exclusive to P3C amplified c-myc was identified slightly 3' of exon one. No alterations in c-myc DNAse I hypersensitive site patterns were observed in HFF fibroblasts following serum release, when peak levels of c-myc transcript were induced. DNAse I hypersensitive site patterns associated with gene amplification may reflect a compensatory response by P3C cells to an abundance of c-myc transcript. Furthermore, elevated levels of protein in P3C cells provide additional evidence that amplified c-myc is an oncogene in MFHs.