Chromatin Immunoprecipitation Assays: Me
β Philippe Collas (auth.), Philippe Collas (eds.)
π Library
π
2009
π Humana Press
π English
β¦ LIBER β¦
π
Chromatin Immunoprecipitation Assays: Methods and Protocols
β Scribed by Philippe Collas (auth.), Philippe Collas (eds.)
- Publisher
- Humana Press
- Year
- 2009
- Tongue
- English
- Leaves
- 268
- Series
- Methods in Molecular Biology 567
- Edition
- 1
- Category
- Library
β¬ Acquire This Volume
No coin nor oath required. For personal study only.
β¦ Synopsis
Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful Methods in Molecular Biologyβ’ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easy-to-use, Chromatin Immunoprecipitation Assays: Methods and Protocols is a timely guide for all those who wish to further the development of this powerful tool and continue delving into the incredible complexity of the cellβs biology.
β¦ Table of Contents
Front Matter....Pages i-x
Back Matter....Pages 1-25
....Pages 27-43
β¦ Subjects
Human Genetics; Microarrays
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