Chromatin-bound DNA polymerase from higher plants

✍ Scribed by Christine Stevens; John A. Bryant; P. Carol Wyvill

• Book ID: 104751126
• Publisher: Springer-Verlag
• Year: 1978
• Tongue: English
• Weight: 702 KB
• Volume: 143
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00389060

✦ Synopsis

Chromatin-bound DNA polymerase has been extracted from pea (Pisum sativum L.) seedlings, and partially purified by solubilization from chromatin followed by chromatography on columns of either DEAE-cellulose or DEAE-Sephadex. The enzyme elutes from DEAE-cellulose as a single peak, but is fractionated into two peaks, CI and CII, by DEAE-Sephadex chromatography. If the enzyme is stored at-15°C for several days prior to chromatography, a third peak, CIII, derived from CII, is obtained. The polymerase is devoid of nuclease activity, and is relatively insensitive to N-ethyl-maleimide. These features, taken with the ion requirements and with data obtained from other plant species, lead to the suggestion that the chromatin-bound DNA polymerase of higher plants is similar to the DNA polymerase-β from vertebrates.