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Chromatin assembly in vitro and in vivo

✍ Scribed by Stephen M. Dilworth; Colin Dingwall

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
833 KB
Volume
9
Category
Article
ISSN
0265-9247

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✦ Synopsis

The assembly of nucleosomes and higherorder chromatin structures has been extensively studied in vitro. Provided that non-specific charge interactions are controlled, all the information for correct assembly is found to be inherent in the macromolecular components. Cellular extracts which can assemble chromatin in vitro with nucleosomes correctly spaced on the D N A have been studied in detail and also used to investigate the role of chromatin structure in transcription. However, the mechanisms of chromatin assembly in vivo are still controversial.

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