Chromate tolerance caused by reduced hydroxyl radical production and decreased glutathione reductase activity in Schizosaccharomyces pombe
✍ Scribed by Zoltán Gazdag; István Pócsi; József Belágyi; Tamás Emri; Ágnes Blaskó; Krisztina Takács; Miklós Pesti
- Book ID
- 102388753
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 139 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Abstract
The stable Cr(VI)‐tolerant chr1‐66T mutant of Schizosaccharomyces pombe, which carries one simple gene mutation responsible for Cr(VI) tolerance, accumulated and reduced the chromate anion (CrO~4~^2–^) significantly more slowly than did its parental strain 6chr^+^. The mutant chr1‐66T proved to be sensitive to oxidative stressors such as H~2~O~2~, menadione, tert‐butyl hydroperoxide and Cd^2+^. Both the Cr(VI) tolerance and the oxidative stress sensitivity were attributed to a decreased specific glutathione reductase activity. These effects were also enhanced with a decrease in the specific mitochondrial Mn‐SOD activity.