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Chondrocyte survival in cryopreserved osteochondral articular cartilage

✍ Scribed by Christian Ohlendorf; William W. Tomford; Henry J. Mankin

Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
485 KB
Volume
14
Category
Article
ISSN
0736-0266

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✦ Synopsis

Abstract

Fluorescent staining techniques were used in combination with confocal and conventional fluorescent microscopy to determine the location of viable chondrocytes in frozen and thawed osteochondral articular cartilage. The results showed that cell survival was confined to the superficial layer of the cartilage matrix. The addition of a cryopreservative agent (dimethyl sulfoxide) increased chondrocyte survival but only in the same layer. There was no evidence of cell survival in the middle or deep layers of the cartilage with or without the use of dimethyl sulfoxide. Under the conditions employed in this study, chondrocyte survival in a cryopreserved osteochondral allograft appears to be limited to the superficial layer of the articula cartilage.

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