Cholinergic-stimulated alkaline phosphatase secretion and phospholipid synthesis in guinea pig seminal vesicles
✍ Scribed by D. H. Lockwood; H. G. Williams-Ashman
- Publisher
- John Wiley and Sons
- Year
- 1971
- Tongue
- English
- Weight
- 816 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Abstract
Incubation of slices of seminal vesicles of the guinea pig with cholinergic drugs led to an enhanced secretion of alkaline phosphatase. Incubation with carbamylcholine also stimulated the incorporation of P^32^ into the phospholipid fraction. Both cholinergic effects required a supply of energy since dinitrophenol was inhibitory. The stimulation of enzyme secretion and phospholipid synthesis by carbamylcholine was completely abolished by atropine. Omission of calcium ions from the incubation medium and pre‐treatment of the slices with ethylenediaminetetraacetic acid (EDTA) caused a marked reduction in alkaline phosphatase secretion induced by carbamylcholine but had no effect on incorporation of P^32^ into phospholipids. Adenergic agents such as epinephrine, norepinephrine and isoproterenol did not influence these two processes. Addition of cyclic AMP, dibutyryl cyclic AMP and a phosphodiesterase inhibitor was also ineffective. The incorporation of P^32^ into the various phospholipids of the seminal vesicle was examined. In the presence of carbamylcholine, there was a marked increase in the P^32^‐specific activities of phosphatidylinositol (nearly 6‐fold) and of phosphatidylserine (about 1.5‐fold). These observations indicate that the guinea pig seminal vesicle, a large hollow organ composed of a single layer of epithelium, is ideally suited for studies concerning the biochemistry of macromolecular secretion.