Cholera toxin-catalysed ADP-ribosylation of erythrocyte proteins: General properties

✍ Scribed by Gill, D. Michael

• Book ID: 102926575
• Publisher: Wiley (John Wiley & Sons)
• Year: 1979
• Tongue: English
• Weight: 679 KB
• Volume: 10
• Category: Article
• ISSN: 0091-7419
• DOI: 10.1002/jss.400100205

✦ Synopsis

Upon incubation of lysed pigeon erythrocytes with NAD, adenosine diphosphate-ribose (ADP-ribose) is incorporated into nuclear poly ADP-ribose and into an unidentified acid-insoluble product of the cytosol. The properties of these incorporations have been examined and a method developed for reducing their amount whilst retaining the sensitivity of the lysate to cholera toxin. This method has allowed the detection and description of a set of cholera toxin-specific ADP-ribose transfers to membrane-bound and soluble proteins under conditions that lead to adenylate cyclase activation.