Chlorophyll-protein and polypeptide composition of Mn-deficient sugar beet thylakoids

✍ Scribed by Javier Abadia; John N. Nishio; Norman Terry

• Book ID: 104613625
• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 443 KB
• Volume: 7
• Category: Article
• ISSN: 0166-8595
• DOI: 10.1007/bf00014677

✦ Synopsis

The chlorophyll-protein and polypeptide composition of manganese deficient and control sugar beet thylakoids was examined using three different detergent-electrophoresis systems. On a per chlorophyll basis, manganese deficiency reduced the amounts of CPa complex (separated by sodium dodecylsulfate (SDS)-polyacrylamide gel electrophoresis), and CP 47 and CP 43 complexes (separated by octylglucoside/SDS-polyacrylamide gel electrophoresis) without decreasing the amounts of light harvesting complexes. Lithium dodecylsulfate/Triton X-100 polyacrylamide gel electrophoresis showed that manganese deficiency decreased several thylakoid polypeptides, including a chlorophyll b containing 30 kilodalton chlorophyll-protein complex, but did not decrease the amounts of 28 and 29 kilodalton light-harvesting chlorophyll b-containing polypeptides.