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Chlorophyll interference with phytochrome measurement

✍ Scribed by Ann M. Jose; Daphne Vince-Prue; Janet R. Hilton

Publisher
Springer-Verlag
Year
1977
Tongue
English
Weight
395 KB
Volume
135
Category
Article
ISSN
0032-0935

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✦ Synopsis

Measurements of phytochrome by A (AA725.s 15 nn0 were completely suppressed at chlorophyll concentrations of the order of 20-40gg g 1 f.wt. in vivo and 37lag cm -3 in vitro, and the readings were reduced by 50% at only 12~tg cm -3 in vitro. At these concentrations of chlorophyll in aqueous methanol, the loss of phytochrome signal in vitro appeared to be due to failure of phytochrome photoconversion rather than to interference with AA measurement by chlorophyll fluorescence in the 725/815 nm measuring beam.

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