Chlorophyll fluorescence changes at high temperatures induced by linear heating of greening barley leaves
✍ Scribed by Petr Ilík; Jan Nauš; Daniel Cikánek; Radko Novotný
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 474 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0166-8595
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✦ Synopsis
A relative decrease of the high temperature part (above 60 ° C) of the chlorophyll fluorescence temperature curve during 3 h to 10 h greening period of barley (Hordeum vulgare L.) leaves was found to be concomitant to a decrease of Chl a/b ratio and to a gradual increase of LHCP/core ratio found by electrophoresis and the ratio of granal to total length of thylakoid membranes. It is suggested that the high temperature part of the fluorescence temperature curve depends inversely on the relative amount of LHC II in thylakoid membranes.
Abbreviations: Chl a(b) -chlorophyll a(b); CPa-chlorophyll a protein complex of PS II; CP 1 -P700 chlorophyll a protein complex of PS I; FP-free pigments; FTC-fluorescence temperature curve; F(T30)-fluorescence intensity at 30 °C; LHC II-light harvesting complex II; LHCP-light harvesting chlorophyll protein; LHCP3 (LHCPm)monomeric form of LHC II; LHCPo-oligomeric form of LHC II complex; M1-first maximum of FTC; M 2second maximum (region) of FTC; PAA-polyacrylamide; PAR-photosynthetically active radiation; PS I(II)-Photosystem I(II); SDS-PAGE-sodium dodecyl sulfate polyacrylamide gel electrophoresis