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Chloride transport and the electrophysiological properties of the isolated skin of the shanny (Blennius pholis L.)

✍ Scribed by Williams, E. M. ;Eddy, F. B. ;Ward, M. R.

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
307 KB
Volume
245
Category
Article
ISSN
0022-104X

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✦ Synopsis

The isolated open-circuited shanny skin exhibited a net chloride efflux of 2.91 pmol h-l. The transepithelial potential was 12 mV (inside + ve). Short circuit current was 60 pA cm2 and transepithelial resistance was 225 61 cm2. When either ouabain or furosemide was added to the inside, or amiloride or 4-acetamido-4'-isothiocyanatostibene-2,2'disulphonic acid (SITS) was added to the outside, the transepithelial potential and short-circuit current were reduced. The results are discussed and a model is presented that describes the possible mechanism for chloride transport across shanny skin.

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