Chloride-dependent pH regulation in connective glial cells of the leech nervous system
β Scribed by Marek S. Szatkowski; Wolf-R. Schlue
- Book ID
- 113127087
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 340 KB
- Volume
- 665
- Category
- Article
- ISSN
- 0006-8993
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π SIMILAR VOLUMES
Double-barrelled pH-sensitive microelectrodes were used to measure the intracellular pH (pH,) of neuropil glial cells and the pH of extracellular spaces (pH,) within isolated, intact ganglia of the leech Hirudo medicinalis. By application of a weak acid (propionate, 40 mM) or a weak base (ammonium,
We used double-barrelled, neutral carrier, pH-sensitive microelectrodes to study the mechanisms by which the intracellular pH (pHJ is regulated in the connective glial cells of the medicinal leech. In HEPES-buffered, nominally C02/HCO;-free solutions the recovery of pHi from intracellular acidosis i
The patch-clamp technique was used to investigate the activity of single ion channels in neuropile glial (NG) cells in the central nervous system (CNS) of the medicinal leech, Hirudo rnediciaulis. We found evidence for two distinct C1-channels that could be distinguished by their basic electrical pr