Chlorella sp. and Arsenic Compounds: An Attempt to Prepare an Algal Reference Material for Arsenic Compounds

✍ Scribed by W. Goessler; J. Lintschinger; J. Száková; P. Mader; J. Kopecký; J. Doucha; K. J. Irgolic

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 209 KB
• Volume: 11
• Category: Article
• ISSN: 0268-2605
• DOI: 10.1002/(sici)1099-0739(199701)11:1<57::aid-aoc540>3.0.co;2-j

✦ Synopsis

The growth of Chlorella Böhm, Chlorella kessleri and Chlorella 108 in media containing sodium arsenate at 200 or 2000 mg As dm Ϫ 3 was investigated. The cell densities in the stationary phases were 40% higher for Chlorella Böhm (5.6ϫ 10 5 cells cm Ϫ 3 ) and Chlorella kessleri (4.5ϫ 10 5 cells cm Ϫ 3 ) grown in the 2000 mg As dm Ϫ 3 ) arsenate media compared with the arsenic-free media. The growth of Chlorella 108 was depressed by arsenate. Dimethylarsinic acid (at concentrations never exceeding 1.2 mg As dm Ϫ 3 ) and arsenite ( ~0.2 mg As dm Ϫ 3 ) were detected in the growth medium. The cells harvested during the stationary phase had 2400 mg arsenic associated with 1 kg dry mass. A water/ methanol (80 : 20) mixture extracted all the arsenic from the cells. Most of the arsenic (99%) was arsenate. Arsenite, methylarsonic acid and dimethylarsinic acid accounted for the remaining arsenic. Cadmium in the arsenic-containing growth media (50 g to 100 mg dm Ϫ 3 ) reduced the yield of algal cells and induced the formation of an unidentified arsenic compound in Chlorella Böhm.