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CHK1 frameshift mutations in genetically unstable colorectal and endometrial cancers

✍ Scribed by Francesco Bertoni; Anna Maria Codegoni; Daniela Furlan; Maria Grazia Tibiletti; Carlo Capella; Massimo Broggini

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 67 KB
Volume: 26
Category: Article
ISSN: 1045-2257
DOI: 10.1002/(sici)1098-2264(199910)26:2<176::aid-gcc11>3.0.co;2-3

No coin nor oath required. For personal study only.

✦ Synopsis

The protein encoded by the CHK1 gene plays an important role in the G2 checkpoint in mammalian cells. In its coding region it presents a sequence of nine consecutive adenines that are a potential site of mutations in tumors with microsatellite instability (MSI). We analyzed the presence of frameshift mutations in the CHK1 gene in human colon and endometrial cancer samples. In the same cancer samples genes known to be altered in these tumors (BAX, TGFBRII, and IGFIIR) were also analyzed. CHK1 frameshfit mutations were found in 1 out 10 colon cancers and 2 out of 7 endometrial cancers showing MSI. CHK1 alterations were associated with the presence of a high degree of MSI. No alterations were found in patients with tumors showing low frequency or lacking instability (microsatellite stable). The same was true for the other four genes analyzed. The insertion or deletion of one A in the poly A tract resulted in a truncated protein. Alterations of the CHK1 gene could represent an alternative way of cancer cells to escape from cell cycle control.

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