Chitosan prevents the development of AOM-induced aberrant crypt foci in mice and suppressed the proliferation of AGS cells by inhibiting DNA synthesis
✍ Scribed by Shyr-Yi Lin; Hing-Yuen Chan; Fung-Hsiu Shen; Mei-Huei Chen; Ying-Jan Wang; Chung-Keung Yu
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 166 KB
- Volume
- 100
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
Abstract
We study the effect of fungal‐derived chitosan on the development of chemical‐induced colonic precancerous lesions in ICR mice and delineate its possible molecular mechanisms. In the 2 weeks preventive experiments, mice fed with a diet containing high molecular weight chitosan (HMWC) had significant fewer aberrant crypt foci formation than those fed with control diet. As the treatment extended to 6 weeks, both low molecular weight chitosan (LMWC)‐ and HMWC‐fed mice contained less aberrant crypt foci when compared to control. However, such effect was not observed in mice in the 6 weeks therapeutic experiments. The anti‐tumorigenesis effect of water‐soluble chitosan oligomer (WSCO) was tested on four cancer cell lines. WSCO significantly suppressed AGS and to a less extent, COLO 205 cells proliferation. Flow cytometry analysis of cell cycle distribution indicated that the percentage of S phase reduced significantly in AGS cells treated with WSCO together with a decrease in DNA synthesis rate in BrdU incorporation assay. WSCO treatment also upregulated cell cycle‐related genes p21/Cip and p27/Kip, whereas downregulated that of PCNA. J. Cell. Biochem. 100: 1573–1580, 2007. © 2007 Wiley‐Liss, Inc.