Chitosan-phosphorylated chitosan polyelectrolyte complex hydrogel as an osteoblast carrier
✍ Scribed by Quan-Li Li; Zhi-Qing Chen; Brian W. Darvell; Lai-Kui Liu; Hong-Bing Jiang; Quan Zen; Qian Peng; Guo-Min Ou
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 372 KB
- Volume
- 82B
- Category
- Article
- ISSN
- 1552-4973
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✦ Synopsis
Abstract
To simulate extra‐cellular matrix, a novel three‐dimensional scaffold of polyelectrolyte complex (PEC) hydrogel as an osteoblast carrier was synthesized. First, chitosan, a natural glycosaminoglycan, was modified by phosphorylation to obtain a water‐soluble phosphorylated chitosan (P‐content: 10.7 mass%). The PEC hydrogel was then formed from equal volumes of 0.173 mass% phosphorylated chitosan in water and 1 mass% chitosan in 1% (V/V) acetic acid solution. Rat osteoblasts were seeded in the hydrogel. The PEC hydrogel had a three‐dimensional hierarchically‐porous structure and good cytobiocompatibility for osteoblasts in vitro. It is concluded that the PEC hydrogel is a promising material as an osteoblast carrier. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2007