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Chitosan Biotinylation and Electrodeposition for Selective Protein Assembly

✍ Scribed by Xiao-Wen Shi; Yi Liu; Angela T. Lewandowski; Li-Qun Wu; Hsuan-Chen Wu; Reza Ghodssi; Gary W. Rubloff; William E. Bentley; Gregory F. Payne

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
219 KB
Volume
8
Category
Article
ISSN
1616-5187

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✦ Synopsis

Abstract

An alternative route to protein assembly at surfaces based on using the unique capabilities of biological materials for the spatially selective assembly of proteins is described. Specifically, the stimuli‐responsive properties of aminopolysaccharide chitosan are combined with the molecular‐recognition capabilities of biotin‐streptavidin binding. Biotinylated chitosan retains its stimuli‐responsive properties and is capable of electrodepositing at specific electrode addresses. Once deposited, it is capable of binding streptavidin, which can mediate the subsequent assembly of biotinylated proteins. Spatially selective protein assembly using biotinylated Protein A and fluorescently‐labeled antibodies is demonstrated.

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