Chiral separation performance of micrometer-sized monodispersed silica spheres with high protein loading
β Scribed by Zheng Zhai; Yang Chen; Yu-Jun Wang; Guang-Sheng Luo
- Book ID
- 102072630
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 252 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0899-0042
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π SIMILAR VOLUMES
Protein A, a bacterial cell wall protein found in Staphylococcus aureus, has been widely used for the analysis of immunoglobulins. By attaching protein A to a microparticulate silica support, a rapid and efficient chromatographic sorbent has been created for the separation of monoclonal antibodies.
Non-porous monodisperse 1.5pm silicas were allowed to react with (A) and (B) N-acetylaminopropyltriethoxysilane to generate bonded phases useful in high-performance hydrophobic-interaction chromatography (HIC). Differences in the selectivity were observed between the amide and the ether phase. Peak