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Chiral separation of the β2-sympathomimetic fenoterol by HPLC and capillary zone electrophoresis for pharmacokinetic studies

✍ Scribed by Thomas Ullrich; Dirk Wesenberg; Corinna Bleuel; Gerd-Joachim Krauss; Martin G. Schmid; Michael Weiss; Gerald Gübitz

Publisher: John Wiley and Sons
Year: 2010
Tongue: English
Weight: 396 KB
Volume: 24
Category: Article
ISSN: 0269-3879
DOI: 10.1002/bmc.1415

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✦ Synopsis

Abstract

The development of methods for the separation of the enantiomers of fenoterol by chiral HPLC and capillary zone electrophoresis (CZE) is described. For the HPLC separation precolumn fluorescence derivatization with naphthyl isocyanate was applied. The resulting urea derivatives were resolved on a cellulose tris(3,5‐dimethylphenylcarbamate)‐coated silica gel column employing a column switching procedure. Detection was carried out fluorimetrically with a detection limit in the low ng/mL range. The method was adapted to the determination of fenoterol enantiomers in rat heart perfusates using liquid–liquid extraction. As an alternative a CE method was used for the direct separation of fenoterol enantiomers comparing different cyclodextrin derivatives as chiral selectors. Copyright © 2010 John Wiley & Sons, Ltd.

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