Chiral discrimination of N-carbazole-carbonyl derivatives of α-amino acids with a short linear alkyl side chain by bovine serum albumin
✍ Scribed by Yoshihiro Abe; Tomoko Shoji; Mamiko Matsubara; Midori Yoshida; Setsuro Sugata; Kazunori Iwata; Hiroshi Suzuki
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 119 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0899-0042
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✦ Synopsis
Chiral discrimination of racemic carbazole carbonyl (CC)-amino acids with linear alkyl sidechain (C 1 -C 4 ) by bovine serum albumin (BSA) was investigated by competitive replacement experiments using dansyl-L-proline and dansyl-D-norvaline as fluorescent probes. It was found that the CC derivatives of the D-forms of alanine (C 1 ), amino butyric acid (C 2 ), norvaline (C 3 ), and norleucine (C 4 ) bound to the dansyl-Lproline site much more strongly than their L-forms, whereas the interactions between both enantiomers of these amino acids with dansyl-D-norvaline site were slight.