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Chip-Based Analysis of Protein−Protein Interactions by Fluorescence Detection and On-Chip Immunoprecipitation Combined with μLC−MS/MS Analysis

✍ Scribed by Sydor, Jens R.; Scalf, Mark; Sideris, Steve; Mao, Guo Dong; Pandey, Yash; Tan, Ming; Mariano, Maria; Moran, Michael F.; Nock, Steffen; Wagner, Peter


Book ID
120240184
Publisher
American Chemical Society
Year
2003
Tongue
English
Weight
237 KB
Volume
75
Category
Article
ISSN
0003-2700

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## Abstract To improve the efficiency of proteome analysis, a strategy with the combination of protein pre‐fractionation by preparative microscale solution isoelectric focusing, peptide separation by μRPLC with serially coupled long microcolumn and protein identification by ESI‐MS/MS was proposed.