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Chinese hamster cells exhibiting a temperature dependent alteration in purine transport

✍ Scribed by J. F. Harris; G. F. Whitmore

Publisher
John Wiley and Sons
Year
1974
Tongue
English
Weight
743 KB
Volume
83
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

This paper reports the isolation of a phenotypically stable line of Chinese hamster ovary cells which exhibits a temperature dependent alteration in the transport of some purines. The alteration manifests itself for the uptake of guanine, hypoxanthine, azaguanine and guanosine but not for adenine, adenosine or thymidine. Studies with crude cell extracts suggest that, at the temperature the alteration is being expressed, the HGPRT activity is within the normal range. In cell‐cell hybridization studies the alteration behaves as a recessive genetic trait.

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