Chemotaxis and the early stages of aggregation in cellular slime molds
β Scribed by Morrel H. Cohen; Anthony Robertson
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 733 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0022-5193
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## T W O FIGURES The Myzothnllophyta, particularly those organisms within the family, Dictyosteliacene, represent remarkable biological material for the study of celluIar differentiation. The life cycles of these forms are now well known, primarily due to the researches of Olive ( '(la), Raper ( '
The cellular slime molds arise by a n aggregation of amocbac, arid the resulting cell mass often will wander some distance over tlie surface of the substratum before rising into the a i r to fruit. The fruiting body consists of a single or branched stalk made up of large vlrcuolate amoebae encased i
Aggregation of Dictyostelium amoebae is inhibited by light. White light intensities >102 laW-cm 2 cause an inhibition which reaches a saturation at 2.103 laW" cm-2. The action spectrum, based on photon fluence -response curves, shows a major peak around 405 nm and extends through most of the visible
The influence of light and different concentrations of ATP on cell aggregation in cyclic AMP sensitive ( Dictyosteliurn mucoroides, D. purpureum) and cyclic AMP insensitive species (Polysphondylium violaceum, P. pallidurn, D. lacteurn) of the cellular slime molds was observed in small and in large a
Cysteine proteinase activities have been determined using gelatin-SDS-PAGE analysis and assays based on peptide nitroanilides. Vegetative myxamoebae of all species examined contain high levels of cysteine proteinose activity present in multiple forms. In both Dictyoste- lium discoideum and Polysphon