Chemosensitivity testing of human solid tumors. A review of 1582 assays with 258 clinical correlations

✍ Scribed by Carl A. Bertelsen; Vernon K. Sondak; Barry D. Mann; Edward L. Korn; David H. Kern

• Book ID: 102668454
• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 593 KB
• Volume: 53
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(19840315)53:6<1240::aid-cncr2820530604>3.0.co;2-y

✦ Synopsis

To improve clinical interpretation and use of in vitro clonogenic assay results, the authors reviewed their experience to date with chemosensitivity testing of over 1500 solid tumors. All clonogenic assays were performed using a double-layer-soft-agar system with continuous exposure of cells to one concentration of standard anticancer drugs. Significant growth was defined as 230 colonies/control plate. Clinical responses were determined according to standard criteria. Data were analyzed using two different criteria of in vitro sensitivity (250% and 275% inhibition of colony formation) and independently for each histologic type of tumor. Overall, 68% of specimens plated produced significant growth in vitro. Cloning ability varied from 57% to 82% depending on tumor histology. The assay was 57% reliable for predicting in vivo sensitivity, and 92% reliable for in vivo resistance. Predictive accuracy for sensitivity varied from 30% to 86%, depending on the tumor histology. Use of 250% ICF (inhibition of colony formation) as criteria for differentiating sensitivity from resistance proved most reliable, although criteria should be individualized for each tumor type to maximize predictive accuracy.