Chemosensitivity testing of fresh leukaemia cells using the MTT colorimetric assay
โ Scribed by Peter R. Twentyman; Norma E. Fox; John K. H. Rees
- Book ID
- 114711784
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 529 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0007-1048
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The results of in vitro chemosensitivity testing using the MTT assay of tumor cells from 140 patients were analyzed with reference to the clinical antitumor effects of the chemotherapy. One hundred and twenty-four (88.6%) of 140 specimens were successfully tested by the method of Mosmann (J Immunol
Numerous procedures have been described which test the chemosensitivity of tumor cell lines. A major disadvantage of most of these assays is that practical limitations prevent the testing of more than a few variables. We have adapted a rapid and efficient colorimetric assay for testing the chemosens