Chemoselective fabrication of high density peptide microarray by hetero-bifunctional tetra(ethylene glycol) linker for click chemistry conjugation
✍ Scribed by Yongfeng Zhao; Yan Liu; Ilkeun Lee; Yang Song; Xiangdong Qin; Francisco Zaera; Jiayu Liao
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 433 KB
- Volume
- 100A
- Category
- Article
- ISSN
- 1549-3296
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✦ Synopsis
Abstract
A hetero‐bifunctional tetra(ethylene glycol) molecule with silane and azide termini was synthesized, and this molecule was used to prepare azide‐derivatized glass surface in one step. The resulting glass surface was available for fabricating peptide microarray by the conjugation with alkyne‐containing peptide using click chemistry, which proceeded to the completion at low temperature and in aqueous solution. A high density of peptide on the surface was achieved due to concise overall procedure and highly efficient conjugation reaction. Immobilized peptides were highly bio‐functional on the surface, as demonstrated by the ability to detect protease activity. Due to the biologically orthogonal manner of conjugation, peptide conjugated by site‐specific immobilization was more accessible by protease than that conjugated by random amide conjugation. This site‐specific and high efficient immobilization technique could be expanded to large scale development of biocompatible peptide and protein arrays for use in various applications. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.