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Chemoenzymatic Transfer of Fluorescent Non-natural Amino Acids to the N Terminus of a Protein/Peptide

✍ Scribed by Masumi Taki; Hiroyuki Kuroiwa; Masahiko Sisido


Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
291 KB
Volume
9
Category
Article
ISSN
1439-4227

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## Abstract We report here a new strategy for derivatizing peptides and proteins at the N‐terminus. To achieve this, a nonnatural amino acid was charged onto a tRNA and then enzymatically transferred to a lysine (Lys) unit at the N‐terminus of a peptide or a protein by using L/F‐tRNA‐protein transf