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Chemoenzymatic Synthesis of a Characteristic Glycophosphopeptide from the Transactivation Domain of the Serum Response Factor

✍ Scribed by Jörg Sander; Herbert Waldmann


Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
91 KB
Volume
38
Category
Article
ISSN
0044-8249

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✦ Synopsis


Glycopeptides, phosphopeptides, and glycophosphopeptides can be synthesized efficiently by a strategy based on a combination of suitable enzyme-labile protecting groups. Thus, probes for biological studies can be accessed. An example is the glycosylated and phosphorylated heptapeptide 1 from the transactivation domain of the human serum response factor, which contains an additional biotin label for detection with streptavidin.


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