Chemoenzymatic Synthesis and Fluorescent Visualization of Cell-Surface Selectin-Bound Sialyl Lewis X Derivatives
✍ Scribed by Valentin Wittmann; Arun K. Datta; Kathryn M. Koeller; Chi-Huey Wong
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 249 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0947-6539
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✦ Synopsis
Sialyl Lewis x (sLe x ) derivatives conjugated to readily visualized molecular labels are useful chemical probes to study selectin ± carbohydrate interactions. Localization of the selectins on the surface of leukocytes and activated endothelial cells can be detected through fluorescence of bound selectin ligands. Herein we present a short chemoenzymatic synthesis of a fluorescently labeled bivalent sLe x conjugate. The use of an amino-substituted monovalent sLe x to obtain fluorescent-and biotin-labeled sLe x derivatives is also described. The cell-staining utility of the fluorescent sLe x conjugates is demonstrated for a HUVEC cell line expressing E-selectin and for CHO-K1 cells expressing either L-or E-selectin.