Chemistry of some fluorescamine–amine derivatives with relevance to the biosynthesis of benzylpenicillin by fermentation
✍ Scribed by W L Baker; G T Lonergan
- Book ID
- 102325619
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2002
- Tongue
- English
- Weight
- 113 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0268-2575
- DOI
- 10.1002/jctb.706
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✦ Synopsis
Abstract
The reaction of fluorescamine with 6‐aminopenicillanic acid (6‐APA) at pH 4 was investigated for its potential use in the biosynthesis of benzylpenicillin. A number of amino acids and penicillin amine derivatives, that reacted with fluorescamine at pH 7–9, were unlikely to do so to a significant extent at pH 4 and hence were unlikely to interfere in results that led to the biosynthesis of benzylpenicillin. Biosynthesis was followed using Penicillium chrysogenum Wis F3‐64, growing in a corn steep liquor medium in a shake flask fed with phenylacetic acid daily. Analysis of benzylpenicillin formation was effected enzymically and fluorimetrically. A sample of the fermentation broth was treated with buffer at pH 7.8 and an active penicillin acylase solution for 1 h at 37 °C. The pH was then lowered to 4 by swamping with acetate buffer and the solution was treated with fluorescamine. The resulting fluorescence was compared with that of a standard 6‐aminopenicillianic acid solution treated in the same manner. In this case the biosynthesis of benzylpenicillin was found to increase over 6 days. The results were compared with those for a control broth where the penicillin acylase was not added.
© 2002 Society of Chemical Industry