ChemInform Abstract: Palladium-Catalyzed Attachment of Labels with Acetylenic Linker Arms to Biological Molecules.

✍ Scribed by G. T. CRISP; J. GORE

• Publisher: John Wiley and Sons
• Year: 2010
• Weight: 25 KB
• Volume: 28
• Category: Article
• ISSN: 0931-7597
• DOI: 10.1002/chin.199719253

✦ Synopsis

Palladium-Catalyzed Attachment of Labels with Acetylenic Linker Arms to Biological Molecules.

-A series of fluorescent and enzymic labels containing a terminal alkyne group linker are attached to suitably derivatized biomolecules, e.g. amino acids, nucleosides and steroids, by Pd-catalyzed coupling. The terminal alkyne group forms a nonpolar covalent bond between the linker arm and the biomolecule and to the position of the label in a remote position from functional groups on the biomolecule. The method allows the detection of biomolecules at concentrations in the subnanogram range. -(CRISP, G. T.;