ChemInform Abstract: Oligosaccharide Synthesis by Coupled endo-Glycosynthases of Different Specificity: A Straightforward Preparation of Two Mixed-Linkage Hexasaccharide Substrates of 1,3/1,4-β-Glucanases.

Oligosaccharide Synthesis by Coupled end

Oligosaccharide Synthesis by Coupled endo-Glycosynthases of Different Specificity: A Straightforward Preparation of Two Mixed-Linkage Hexasaccharide Substrates of 1,3/1,4-β-Glucanases

✍ Magda Faijes; Jon K. Fairweather; Hugues Driguez; Antoni Planas 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 111 KB

Glycosynthases are engineered glycosidases which are hydrolytically inactive yet efficiently catalyse transglycosylation reactions of glycosyl fluoride donors, and are thus promising tools for the enzymatic synthesis of oligosaccharides. Two endo-glycosynthases, the E134A mutant of 1,3/1,4-bglucanas