ChemInform Abstract: Modulation of the Rate, Enantioselectivity, and Substrate Specificity of Semisynthetic Transaminases Based on Lipid Binding Proteins Using Site Directed Mutagenesis.
✍ Scribed by H. KUANG; R. R. DAVIES; M. D. DISTEFANO
- Publisher
- John Wiley and Sons
- Year
- 2010
- Weight
- 33 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0931-7597
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✦ Synopsis
Modulation of the Rate, Enantioselectivity, and Substrate Specificity of Semisynthetic Transaminases Based on Lipid Binding Proteins Using Site Directed Mutagenesis.
-New intestinal fatty acid binding protein-pyridoxamine conjugates ( IFABP-PX), in which the position of the pyridoxamine attachment is varied by site directed mutagenesis, are synthesized and applied to reductive amination reactions of several α-keto acids. The results indicate, that site-directed mutagenesis can be used to tune the rate, enantioselectivity, and substrate specificity of semisynthetic transaminases based on fatty acid binding proteins.
-(KUANG, H.;