Chemiluminescence investigations of antioxidative activities of some antibiotics against superoxide anion radical

✍ Scribed by Irena Kruk; Teresa Michalska; Aleksandra Kładna; Paweł Berczyński; Hassan Y. Aboul-Enein

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 755 KB
• Volume: 26
• Category: Article
• ISSN: 1522-7235
• DOI: 10.1002/bio.1278

✦ Synopsis

A chemiluminescent technique was applied to determine antioxidative activities of adriamycin, farmorubicin, mitomycin C and bleomycin against superoxide anion radical (O 2

• ) in aprotic medium. The antioxidant capacity was expressed as the decrease in light emission from the O 2

• solution by and antibiotic. A KO 2 solution in dimethyl sulphoxide (DMSO) and 18-crown-6 ether were used for the generation of O 2

• . The results showed that the examined compounds decreased the chemiluminescence (CL) sum from the O 2 • -generating system in a dose-dependent manner. Among the antibiotics examined, adriamycin, farmorubicin and bleomycin exhibited antioxidant activity almost comparable to that of 1,2-dihydroxy benzene-3,5-disulphonic acid (tiron), an efficient of the O 2 • inhibitor. Mitomycin C was two-times less effective as tiron in decreasing the initial CL intensity. The proposed assay with usage of ultraweak CL technique and the KO 2 -DMSO-crown ether system was useful for the evaluation of antioxidant activity in aprotic solvents.