Chemiluminescence determination of chlorpheniramine using tris(1,10-phenanthroline)–ruthenium(II) peroxydisulphate system and sequential injection analysis

✍ Scribed by Fakhr Eldin O. Suliman; Mohammed M. Al-Hinai; Salma M. Z. Al-Kindy; Salama B. Salama

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 266 KB
• Volume: 24
• Category: Article
• ISSN: 1522-7235
• DOI: 10.1002/bio.1054

✦ Synopsis

A sequential injection (SI) method was developed for the determination of chlorpheniramine (CPA), based on the reaction of this drug with tris(1,10-phenanthroline)-ruthenium(II) [Ru(phen) 3 2+ ] and peroxydisulphate (S 2 O 8 2-) in the presence of light. The instrumental set-up utilized a syringe pump and a multiposition valve to aspirate the reagents [Ru(phen) 3 2+ and S 2 O 8 2-

] and a peristaltic pump to propel the sample. The experimental conditions affecting the chemiluminescence reaction were systematically optimized, using the univariate approach. Under the optimum conditions linear calibration curves of 0.1-10 mg/ml were obtained. The detection limit was 0.04 mg/ml and the relative standard deviation (RSD) was always < 5%. The procedure was applied to the analysis of CPA in pharmaceutical products and was found to be free from interferences from concomitants usually present in these preparations.