Chemically Modified PPyCOOH Microtubes as an Affinity Matrix for Protein Purification
✍ Scribed by Wei Shi; Huihui Cao; Yuqing Shen; Cunfeng Song; Donghui Li; Ye Zhang; Dongtao Ge
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 321 KB
- Volume
- 210
- Category
- Article
- ISSN
- 1022-1352
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✦ Synopsis
Abstract
Novel affinity microtubes, with high ligand content and adsorption capacity of proteins, were fabricated by functionalization of the carboxyl group on the inner and outer surfaces of conductive polymer, poly(1‐(2‐carboxyethyl)pyrrole) (PPyCOOH) microtubes with iminodiacetic acid (IDA), which affords the active site to form metal‐IDA chelates used for immobilized‐metal affinity chromatography. After immobilization with copper ions, the microtubes were used to recover a model protein, bovine hemoglobin, from red cell lysate in a batch manner. The protein adsorption indicates that the affinity microtubes exhibit a high adsorption capacity and good selectivity, and the adsorption isotherm fitted the Freundlich model well. Elution of the protein showed that the desorption ratio was up to 99.5% in 3 h, using 0.2 M CH~3~COOHCH~3~COONa buffer solution (pH = 3.6) as the desorption agent. The adsorption‐desorption cycle was repeated 10 times using the same microtubes without significant loss in the hemoglobin adsorption capacity.
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