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Chemically defined medium for the production of biologically active substances of CHO cells

✍ Scribed by Jun-ichiro Hata; Takeyoshi Tamura; Soji Yokoshima; Shinya Yamashita; Shoko Kabeno; Ken Matsumoto; Kazukiyo Onodera

Publisher
Springer
Year
1992
Tongue
English
Weight
390 KB
Volume
10
Category
Article
ISSN
0920-9069

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✦ Synopsis

A recombinant CHO cell line (GT19) secreting a high level of human growth hormone (hGH) was constructed with amplification of the introduced hGH gene. The cells grew well in the alpha MEM medium supplemented with 5% dialyzed fetal calf serum (dFCS), but not with less than 1% dFCS. Therefore we examined various medium components and obtained an improved medium which supported cell growth at low serum concentrations. The production of hGH by the cells was also enhanced in this medium.

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