Chemical synthesis of yeast mitochondrial ATP synthase membranous subunit 8

✍ Scribed by M. Goetz; J.-M. Schmitter; S. Geoffre; E.J. Dufourc

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 89 KB
• Volume: 5
• Category: Article
• ISSN: 1075-2617
• DOI: 10.1002/(sici)1099-1387(199906)5:6<245::aid-psc187>3.0.co;2-q

✦ Synopsis

Chemical synthesis of highly hydrophobic peptides and proteins remains a challenging problem. Strong interchain associations within the peptide -resin matrix have to be overcome. A synthetic strategy for solid phase peptide synthesis is proposed, mainly based on prolonged coupling time using aprotic polar solvent mixtures. A tailored chromatographic purification was required to obtain a sample sufficiently pure for structural analysis. In this work, the total chemical synthesis of the membrane-embedded yeast mitochondrial ATP synthase subunit 8 is described. The quality of the synthetic protein was checked by electrospray mass spectrometry, its tendency to adopt h-helical secondary structure is evidenced by circular dichroism spectroscopy.