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Chemical Synthesis of Lymphotactin: A Glycosylated Chemokine with a C-Terminal Mucin-Like Domain

✍ Scribed by Lisa A. Marcaurelle; Laura S. Mizoue; Jill Wilken; Lisa Oldham; Stephen B. H. Kent; Tracy M. Handel; Carolyn R. Bertozzi

Publisher: John Wiley and Sons
Year: 2001
Tongue: English
Weight: 297 KB
Volume: 7
Category: Article
ISSN: 0947-6539
DOI: 10.1002/1521-3765(20010302)7:5<1129::aid-chem1129>3.0.co;2-w

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✦ Synopsis

The synthesis of a 93-residue chemokine, lymphotactin, containing eight sites of O-linked glycosylation, was achieved using the technique of native chemical ligation. A single GalNAc residue was incorporated at each glycosylation site using standard Fmoc-chemistry to achieve the first total synthesis of a mucin-type glycoprotein. Using this approach quantities of homogeneous material were obtained for structural and functional analysis.

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